Read e-book online Circulating Tumor Cells PDF

By Richard J. Cote, Ram Datar

This quantity offers the most recent study on circulating tumor cells aimed for melanoma researchers, scientists, and molecular oncologists. It provides the fundamental ideas in the back of circulating tumor cells (CTCs), metastatic biology, and strength functions as to how CTCs can be utilized in diagnostic biomarkers.

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Gene expression analysis at the mRNA level can be achieved on chip via RNA in situ hybridization (RNA ISH) as demonstrated by HB-CTC Chip to analyze human pancreatic CTC expression of Wnt2 [25]. Off-chip mRNA analysis for products of tumor-specific genes can be analyzed by qRT-PCR or RNA-seq methods from pooled CTCs as demonstrated by data 2 Affinity-Based Enrichment of Circulating Tumor Cells 23 obtained from CTC enrichment methods such as GEDI, GO nanosheets, and HB-CTC Chip [11, 17, 25]. However, due to contaminant leucocytes and other blood components captured nonspecifically by these affinity-based CTC enrichment methods, performing high-throughput mRNA analysis on both tumor and non-tumorspecific genes requires further isolation of pure population of CTCs in the post-capture process.

Gertler R et al (2003) Detection of circulating tumor cells in blood using an optimized density gradient centrifugation. In: Allgayer H (ed) Molecular staging of cancer. Springer, Berlin, pp 149–155 5. Becker FF et al (1995) Separation of human breast cancer cells from blood by differential dielectric affinity. Proc Natl Acad Sci U S A 92(3):860–864 6. de Bono JS et al (2008) Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res 14(19):6302–6309 7.

EpCAM is a transmembrane glycoprotein that is being expressed in cancers of epithelial origin, and not in normal components in circulation. Thus, antibodies against EpCAM can be used to selectively capture epithelial CTCs from peripheral blood. Here, we discuss several types of affinity-based technologies utilizing EpCAM or other tumor specific antigens in the capture of CTC cells (CellSearch and MagSweeper), as well as technologies that combine affinity-based capture with other physical parameters such as cell size (CTC-Chip and GEDI).

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