By S. M. Fischer, T. J. Slaga (auth.), Susan M. Fischer, Thomas J. Slaga (eds.)
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Extra resources for Arachidonic Acid Metabolism and Tumor Promotion
For methodology see ref. 27. treatment of guinea pig adult primary basal keratinocytes, arachidonic acid and PGF2a are released into the medium after a lag phase of 30 minutes (Fig. 3A,B). All three cultures respond to TPA treatment by an induction of ODC activity. Indomethacin inhibits TPA-induced prostaglandin rel ease and, at the same time, enhances the rel ease of arachi doni c aci d into the culture medium (Fig. 3A,B). In contrast to the situation in mouse epidermis in vivo (15), TPA-induced ODC-activity is not inhibited by indomethacin treatment of the three cell cultures.
Nelson. • and Major. S. Studies on the mechanism of action of anti -tumor promoti ng agents: Thei r speci fi city in two-stage promotion. 6. Proc. Nat1. Acad. Sci. USA 77:2251-2254. 1980. Slaga. • Fischer. M •• Viaje. A•• Berry. L •• Bracken. • LeClerc. S •• and Miller. L. Inhibition of tumor promotion by anti-inflammatory agents: An approach to the biochemical mechanism of promotion. J. Slaga. A. K. ). Carcinogenesis Vol. 2. Mechanisms of TUmor Promotion and 7. Cocarcinogenesis, Raven Press, New York, 1978, p.
Cancer Marks. , Furstenberger, G•• and Richter, H. In: E. Hecker, F. Marks, N. J. Y •• 1982, pp. 331-246. 10. Schwartz. , Slaga. , Hennings, H•• and U. Lichti. F1uocino10ne acetonide: A potent inhibitor of mouse skin tumor promotion and epidermal DNA synthesis. Chem. Bio1. Interact . :331. 1977. 11. Hong. L. and Levine, L. Inhibition of arachidonic acid release from cells as the biochemical action of anti-inflammatory corti- 12. costeroids. Proc. Natl. Acad. Sci. 73:1730. 1976. Gryg1ewski. J. Effects of anti-inflammatory steroids on arachidonate cascade.