Arachidonic Acid Metabolism and Tumor Promotion - download pdf or read online

By S. M. Fischer, T. J. Slaga (auth.), Susan M. Fischer, Thomas J. Slaga (eds.)

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For methodology see ref. 27. treatment of guinea pig adult primary basal keratinocytes, arachidonic acid and PGF2a are released into the medium after a lag phase of 30 minutes (Fig. 3A,B). All three cultures respond to TPA treatment by an induction of ODC activity. Indomethacin inhibits TPA-induced prostaglandin rel ease and, at the same time, enhances the rel ease of arachi doni c aci d into the culture medium (Fig. 3A,B). In contrast to the situation in mouse epidermis in vivo (15), TPA-induced ODC-activity is not inhibited by indomethacin treatment of the three cell cultures.

Nelson. • and Major. S. Studies on the mechanism of action of anti -tumor promoti ng agents: Thei r speci fi city in two-stage promotion. 6. Proc. Nat1. Acad. Sci. USA 77:2251-2254. 1980. Slaga. • Fischer. M •• Viaje. A•• Berry. L •• Bracken. • LeClerc. S •• and Miller. L. Inhibition of tumor promotion by anti-inflammatory agents: An approach to the biochemical mechanism of promotion. J. Slaga. A. K. ). Carcinogenesis Vol. 2. Mechanisms of TUmor Promotion and 7. Cocarcinogenesis, Raven Press, New York, 1978, p.

Cancer Marks. , Furstenberger, G•• and Richter, H. In: E. Hecker, F. Marks, N. J. Y •• 1982, pp. 331-246. 10. Schwartz. , Slaga. , Hennings, H•• and U. Lichti. F1uocino10ne acetonide: A potent inhibitor of mouse skin tumor promotion and epidermal DNA synthesis. Chem. Bio1. Interact . :331. 1977. 11. Hong. L. and Levine, L. Inhibition of arachidonic acid release from cells as the biochemical action of anti-inflammatory corti- 12. costeroids. Proc. Natl. Acad. Sci. 73:1730. 1976. Gryg1ewski. J. Effects of anti-inflammatory steroids on arachidonate cascade.

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