By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)
The annual assembly of the eu Tissue tradition ., Society was once held on the fortress of Zinkovy in Czechoslovakia from might 7-10,1969. incorporated as a part of this assembly was once a symposium on "Aging in telephone and Tissue Culture." This quantity comprises the papers awarded at that symposium. using mobile and tissue tradition thoughts to review the mechanism of getting older isn't new. for instance, it has lengthy been identified that age-associated alterations which happen in plasma can inhibit cellphone proliferation in vitro; additionally that the time lapse ahead of phone migration from ex planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, realization has been thinking about the incidence of senescence in vitro. those investi gations have incorporated experiences of adjustments in non dividing phone cultures, and to a a bit larger volume, of age-related adjustments within the proliferative ability of cells in vitro. for instance, cells derived from human fetal lung hold many houses of ordinary cells together with a strong basic diploid karyotype and those cultures were proven to have a restricted life-span in vitro. In addi tion, cultures derived from human grownup lung exhibit an identical general features and seem to have a shorter existence span than cells derived from fetal lung.
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Extra info for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969
More population doublings were obtained at a higher split ratio. This phenomenon suggests that processes of interaction or selection of cells occur under confluent conditions. A second observation is that the lifespan of human skin fibroblasts in vitro depends upon the type of serum used, heterologous serum being more harmful than homologous or autologous serum [3J. This finding is correlated with changes in the size distribution of the cells and suggests the induction of instability into the cells by the environment.
M. Simons, "The use of frequency distributions of cell diameters to characterize cell populations in tissue culture," Exp. , 45 :336, 1967. 4. M. " To be published. 5. H. V. Tuscany, Personal communication. 39 CHANGES OF THE LATENT PERIOD OF EXPLANTED TISSUES DURING ONTOGENESIS Milena Soukupova, Emma Hole~kova and Premysl HnevkovskY Department of General Biology, Medical Faculty, Charles University, Prague, Czechoslovakia During the first fifty years of work with animal tissues and cells cultured in Vitro, it has been clearly shown that the age of the donor determines the behavior of the explants.
24 R. HAY 15. H. G. Coon, "An established cell line of fibrobroblasts from goose cells, " Carnegie Institution Year Book, 67: 421, 1969. 16. G. J. Todaro, S. R. Wolman, and H. Green, "Rapid transformation of human fibroblasts with low growth potential into established lines by SV40," J. Cell. , 62: 257, 1963. 17. G. J. Todaro and H. Green, "Serum albumin supplemented medium for long-term cultivation of mammalian fibroblast strains," Froc. Soc. Expti. BioI. , 116: 688, 1964. 18. K. H. Rothfels, E.